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Lecturer(s)
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Slavík Martin, Mgr. Ph.D.
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Šlamborová Irena, doc. Mgr. Ph.D.
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Štrojsová Martina, RNDr. Ph.D.
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Course content
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1. Introduction to microscopy and laboratory protocol development. Microscopy of unstained and stained epidermal cells of kitchen onion (Allium cepa). 2. Microscopy of vacuoles in bird's-beak berry (Ligustrum vulgare) cells at different pH, microscopy of cellular inclusions of podding (Tradescantia sp.) and chloroplasts in cells of the meerkat (Mnium sp.). 3. Microscopic observation of fern spores and pollen grains of gymnosperms (monocotyledonous and dicotyledonous) and microscopic observation of cross-section of seedling of tulip (Tulipa sp.). 4. Microscopic observation of various species of cyanobacteria and algae from live cultures and observation of budding yeast (Saccharomyces cerevisiae) under an immersion microscope. 5. Staining and immersion observation of bacteria. 6. Soil microflora. 7. Microorganisms in air, water and on skin. 8. Cultivation of selected fungal strains.
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Learning activities and teaching methods
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Laboratory work, Practicum
- Class attendance
- 54 hours per semester
- Preparation for credit
- 28 hours per semester
- Preparation for exam
- 40 hours per semester
- Class attendance
- 16 hours per semester
- Preparation for credit
- 48 hours per semester
- Preparation for exam
- 50 hours per semester
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Learning outcomes
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Practical exercises aimed at familiarizing and learning to work with the microscope and making various microscopic and microbiological preparations.
Theoretical knowledge and practical skills in the field.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Combined examination, Written assignment, Test
During the semester 2x written test included in the exam. Credit will be awarded for the elaboration of laboratory protocols from each exercise.
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Recommended literature
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Jurčák, J. Základní praktikum z botanické mikrotechniky a rostlinné anatomie.. 1. vyd. Olomouc: PřF UP, 1998.
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Knoz, J., Opravilová, V. Základy mikroskopické techniky. MU, Brno, 1992.
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