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Lecturer(s)
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Rysová Miroslava, Ing. Ph.D.
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Course content
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Lectures: 1. Introduction to applications of nanomaterials for biomedicine and specifications of their use 2. Characterization of living organism. Macromolecules, cell organization, organ systems, organism functions 3. Distribution of nanomaterials for biomedical applications and their functionalization 4. Nanoparticles - biosensors, quantum dots 5. Nanoparticles - drug delivery, carbon nanotubes, graphene 6. Nanoparticles - for hyperthermia, photodynamic therapy, tissue engineering, imaging and contrast agents 7. Polymers and their use for biomedical applications 8. Nanofibers - supports for therapeutic agents, wound dressing 9. Nanofibers - tissue engineering 10. 3D printing. Safety risks and methods for testing of nanomaterials for biomedical applications Tutorials: 11. Nanomaterials production - nanofiber carrier for biomolecules (NanoSpider), nanoparticles, 3D printing 12. Imaging methods for nanomaterials analysis - scanning electron microscopy, dispersion analyser of colloid systems (Zetasizer) 13. Surface modification of nanomaterials - use of plasma 14. Surface modification of nanomaterials - silylation (incorporation of functional groups) 15. Quantification methods for evaluating the effectiveness of surface treatment - fluorescent marking, principle and use of spectrophotometer, calibration line of fluorescence depending on the concentration of a fluorescent substance 16. Quantification of functional groups on the surface of nanomaterial 17. Testing cytotoxicity of nanomaterials for bioapplications - principles of various tests, test preparation 18. Testing cytotoxicity of nanomaterials for bioapplications - cytotoxicity test evaluation 19. Testing activity of nanomaterials with biologically active substance - principles of antimicrobial activity evaluation, test preparation 20. Testing effect of nanomaterials with active substances - test evaluation
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Learning activities and teaching methods
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Monological explanation (lecture, presentation,briefing), Dialogue metods(conversation,discussion,brainstorming), Laboratory work
- Preparation for credit
- 20 hours per semester
- Preparation for exam
- 40 hours per semester
- Semestral paper
- 50 hours per semester
- Class attendance
- 40 hours per semester
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Learning outcomes
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The course is focused on the use of nanomaterials in the context of living organism, transport of biologically active substances and diagnostic and therapeutic nanosystems. Nanodiagnostics, nanopharmacy and nanopharmacology, nanomedicine in selected medical disciplines. Types of nanomaterials used in medicine (quantum dots, nanoparticles, nanofibers). Surface biofunctionalization for bioengineering (production, regeneration and tissue reconstruction) and for biosensors (modification of surfaces reacting on a presence of selected molecules).
Students get an overview of nanomaterials used for biomedical applications. They will have knowledge about types of these nanomaterials and their properties, which determine them for each application. During laboratory exercises students will gain knowledge about production of nanomaterials, testing their effect and cytotoxicity. They will become acquainted with basic analytical and imaging methods used in this area.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Combined examination
The credit requirement is completion of all laboratory exercises. Lecture attendance is not compulsory. The exam is written and oral.
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Recommended literature
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Dinesh, K.B. Krishnakumar, K. Nanofibers: Wound Care Management and Medical Applications. LAP Lambert Academic, 2012.
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Fazel-Rezai, R. Biomedical Engineering - From Theory to Applications. InTech, 2011.
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Fojtík, A. et al. NANO - fascinující fenomén současnosti: nanočástice, nanostruktury a nanotechnologie-důmyslné formy hmoty. Comtes FHT, 2014.
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Ju, H. Zhang, X. Wang, J. NanoBiosensing - Principles, Development and Application. Springer, 2011.
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Rosypal, S. a kol. Nový přehled biologie. Scientia, 2003.
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Thomas, S. Grohens, Y. Ninan, N. Nanotechnology Applications for Tissue Engineering. Elsevier, 2015.
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Webster, T.J. Safety of Nanoparticles: From Manufacturing to Medical Applications. Springer, 2009.
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