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Lecturer(s)
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Horáková Jana, doc. RNDr. Ph.D.
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Course content
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A) Cell and molecular biology (Alberts B.) Introduction to cell biology (characterisation and classification of the cells); chemical composition of the cell (proteins, nukleic acids), cellular structure and function of cell organelles, cell membrane and transport mechanisms across membranes, cell signalling, cell cycle, (cell division, differentiation, cell death, cell cultures, stem cells, tissues, extracellular matrix (composition and function) B) Tissue engineering (Ratner B.D., Lanza R.P., Saltzman W.M.) Fundamental principles of tissue engineering (scaffolds, cells, signals); objectives and processes of tissue engineering, cell material interactions: protein adsorption, focal adhesions (Bacakova L.); wound healing process (Agren M.); experimental in vitro techniques: viability assays, microscopical techniques (Freshney I.E.), in vivo testing (animal models) C) Materials and technologies (Ratner B.D., 2013, Lanza R.P. 2020) Types of biomaterials, scaffold fabrication techniques: textile / non-textile manufacturing methods; material properties influencing cell adhesions and methods for their characterization; drug delivery systems
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Learning activities and teaching methods
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Monological explanation (lecture, presentation,briefing), Dialogue metods(conversation,discussion,brainstorming), Self-study (text study, reading, problematic tasks, practical tasks, experiments, research, written assignments), Independent creative and artistic activities
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Learning outcomes
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The objective of this course is to provide an overview of the principles, materials, and procedures used in the field of tissue engineering. Tissue engineering combines cells, engineering technologies, materials, and appropriate biochemical and physicochemical factors to replace or improve the condition of damaged biological tissue. It is also defined as the study of the principles of tissue growth and the application of this knowledge to create functional tissue replacements for clinical use. Current developments and new findings in biomaterials, stem cells, and biologically active molecules enable tissue regeneration and replacement using so-called "tissue engineering scaffolds." The primary role of scaffolds is to mimic the natural extracellular matrix (ECM). While the extracellular matrix is tissue-specific, its foundation in all tissues is a nano-fibrous structure (comprising fibrous proteins such as collagen and elastin). Due to their morphology, tissue scaffolds based on nano- and micro-fibrous materials hold great potential in this field. Nanofibrous scaffolds offer a high active surface area and a porous structure that facilitates mass transport, while their structure supports cellular adhesion and proliferation. Furthermore, these scaffolds can be chemically and physically modified. The examination for this course consists of two parts. The first part involves presenting a written assignment (thematically related to the student's dissertation thesis). In the second part, the student must demonstrate knowledge based on the exam topics as defined in the syllabus.
The student will acquire detailed knowledge of the subject in the area according to the approval of the Branch Board.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam, Written assignment
A presentation of the written assignment followed by an oral exam in front of a committee appointed by the Dean. The student must demonstrate comprehensive knowledge of the topics defined in the course syllabus.
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Recommended literature
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AGREN, Magnus S. Wound healing biomaterials. Volume 1, Therapies and regeneration. Woodhead Publishing series in biomaterials.. Amsterodam, 2016. ISBN 978-1-78242-455-0.
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Alberts B, et al. Molecular Biology of the cell. 5th ed.. 2008. ISBN 978-0-8153-4110-9.
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Alberts, B. et al. Základy buněčné biologie, úvod do molekulární biologie buňky. Espero Publishing, Ústí nad Labem, 2006. Ústí nad Labem, 2006. ISBN 80-902906-2-0.
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Horáková J., Hujerová M., Šídová A., Erben J. Tkáňové inženýrství. Technická univerzita v Liberci, 2025. ISBN 978-80-7494-746-9.
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Lanza R.P., Langer R., Vacanti J. Principles of tissue engineering, Second edition, Academic Press, 1997.
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NEČAS, Oldřich. Obecná biologie pro lékařské fakulty. 3. přeprac. vyd., V nakl. H & H 1. vyd. Jinočany: H & H, 2000. ISBN 80-86022-46-3. ISBN 80-86022-46-3.
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RATNER, Buddy. Biomaterials Science. 3rd Edition.. Academic Press, 2013. ISBN 978-0-12-374626-9.
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SALTZMAN, Mark W. Tissue Engineering. Oxford University Press, 2004. ISBN 9780195141306.
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ŠEBEK, Jaroslav. Buněčné kultury v medicíně. Praha: Galén, 2018.. ISBN 978-80-7492-380-7.
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