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Lecturer(s)
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Honzíková Petra, Ing.
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Müllerová Senta, Ing.
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Horáková Jana, doc. RNDr. Ph.D.
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Klíčová Markéta, Ing.
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Levitskaya Venera, Mgr.
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Course content
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Lectures: A) Introduction to tissue engineering, cell biology, cell material interactions 1. Introduction to tissue engineering 2. Biomaterials 3. Cell structure and functions 4. Cell cultures 5. Blood cells and thein utilization in tissue engineering 6. Cell material interactions B) In vitro and in vivotesting of tissue engineering scaffolds 7. In vitro testing I 8. In vitro testing II 9. In vivo testing 10. Legislation of medical devices C) Applications of tissue engineering 11. Wound dressings 12. Synthetic vascular prosthesis 13. Materials for prevention of anastomotic leakage 14. Materials for glaucoma treatment Practical courses: 1. Optical microscopy, cell counting, subculturing. 2. Measurement of cell viability using metabolit tests, assessment of cytotoxicity. 3. In vitro testing of biomaterials I: Preparation of materials, sterilization and cell seeding. 4. In vitro testing II: Evaluation of cell proliferation on tested materials 1 week after cell seeding. 5. In vitro testing III: Evaluation of cell proliferation on tested materials 2 weeks after cell seeding. 6. In vitro testing IV: Microscopical analysis of seeded biomaterials. 7. Presentation of achieved results of in vitro testing.
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Learning activities and teaching methods
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Monological explanation (lecture, presentation,briefing), Self-study (text study, reading, problematic tasks, practical tasks, experiments, research, written assignments), Laboratory work, Individual consultation, E-learning
- Class attendance
- 56 hours per semester
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Learning outcomes
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Master course "Materials for tissue engineering" is focused on biomaterials and their interactions with cells and tissues.
Student acquire knowledges of subject.
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Prerequisites
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Unspecified
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Assessment methods and criteria
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Oral exam, Written exam
Credit: Participation on seminars Exam: writtten + oral
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Recommended literature
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Alberts B, et al. Základy buněčné biologie, Espero publishing, Ústí nad Labem, 1998.
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Freshney I. R. Culture of Animal Cells, John Wiley & Sons, Inc., 2010. ISBN 978-0-470-52812-9.
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Gary E. Encyclopedia of Biomaterials and Biomedical Engineering, Marcel Dekker, New York, 2004.
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Lanza R.P., Langer R., Vacanti J. Principles of tissue engineering, Second edition, Academic Press, 1997.
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Peter X. Ma. Scaffolds in Tissue Engineering, Taylor and Francis, New York, 2006.
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RATNER, Buddy. Biomaterials Science. 3rd Edition.. Academic Press,, 2013. ISBN 978-0-12-374626-9.
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