Use of light microscope, various techniques of light microscopy, use of SEM microscopy, preparation of various types of slides, simple image analysis, implementation of a basic biological experiment and its evaluation. <ol> <li>Introduction - construction of light microscope, microscopy practice, SEM microscope principle, confocal microscope, fluorescence microscope. </li> <li>Documentation of microscopic objects - drawing, measuring objects, counting cells (Bürger chamber). </li> <li>Slanted lighting techniques, phase contrast, shadows, fluorescence. </li> <li>Botanical technique - making native preparations (Tradescantia + leaf skin imprints, preparation of cross-sections of leaves and stems of monocotyledonous and dicotyledonous plants). </li> <li>Botanical technique - making simple native preparations - pollen grains, Mnium (chloroplasts), chromoplasts, starch grains, fern spores - drawing practice. </li> <li>Botanical technique - basic procedures for staining botanical native and permanent preparations. </li> <li>Microrelief method, vents and their arrangement, trichomes, determination of leaf area (simple image analysis). </li> <li>Bacteria - making native preparation, possibilities of staining and evaluation, preparation of permanent stained preparations. </li> <li>Yeast - making native preparation, staining, observation, drawing. </li> <li>Zoological technique - making native specimens, vital staining, water-miscible sealing media, observation and documentation of microscopic objects. </li> <li>Permanent preparations - sampling, fixation methods, sections, staining methods. </li> <li>Permanent preparations - spreads, water-immiscible sealable media. </li> <li>Observation of permanent preparation, scale drawings. </li> <li>Basic cytological methods - making pressure preparation, study of individual phases of the cell cycle. </li> </ol>
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Jurčák, J. Základní praktikum z botanické mikrotechniky a rostlinné anatomie.. 1. vyd. Olomouc: PřF UP, 1998.
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Knoz, J., Opravilová, V. Základy mikroskopické techniky. MU, Brno, 1992.
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