|
Lecturer(s)
|
-
Jenčová Věra, doc. Ing. Ph.D.
-
Strnadová Kateřina, Mgr. Ph.D.
|
|
Course content
|
Lectures: 1. Introduction to tissue engineering. 2. Materials for tissue engineering. 3. Morphology of materials (porosity, 2D vs. 3D materials, evaluation methods). 4. Mechanical properties (biomechanics of tissues and scaffolds, measurement methods). 5. Surface properties. 6. Biocompatibility of materials. 7. Cytotoxicity tests. 8. Microscopic techniques. 9. Metabolic tests, DNA analysis. 10. Interaction of materials with proteins. 11. In vivo testing. 12. Biodegradability of materials (degradable materials, degradation mechanisms). 13. Methods of evaluating the degradability of materials. 14. Legislation of medical devices Exercises: Laboratory exercises are focused on practical exercises and verification of students' knowledge acquired during the course lectures and studies of recommended literature. Cell culture, cytotoxicity tests, biocompatibility tests (MTT test, fluorescence microscopy), protein adsorption, degradation of polyester materials.
|
|
Learning activities and teaching methods
|
Self-study (text study, reading, problematic tasks, practical tasks, experiments, research, written assignments), Laboratory work, Problematic methods (research and exploration)
- Class attendance
- 56 hours per semester
- Preparation for laboratory testing; outcome analysis
- 40 hours per semester
- Preparation for exam
- 40 hours per semester
|
|
Learning outcomes
|
The course aims to provide students with an overview of laboratory methods used to evaluate materials developed for tissue engineering. The lectures and exercises will discuss methods related to the evaluation of biocompatibility of materials, physical properties of materials and their biodegradability. The course will provide information about the methods (characterization of materials) used to evaluate the properties of materials for their future applied use. Laboratory exercises are focused on practical practice of basic methods used in practice and knowledge that students have acquired during lectures and studies of recommended literature.
Theoretical knowledge and practical skills in the field.
|
|
Prerequisites
|
unspecified
|
|
Assessment methods and criteria
|
Combined examination
Credit: Laboratory exercise 100% participation, elaboration of protocols.
|
|
Recommended literature
|
-
Alberts B, et al. Molecular Biology of the cell. 5th ed.. 2008. ISBN 978-0-8153-4110-9.
-
Alberts, B. et al. Základy buněčné biologie, úvod do molekulární biologie buňky. Espero Publishing, Ústí nad Labem, 2006. Ústí nad Labem, 2006. ISBN 80-902906-2-0.
-
Freshney I. R. Culture of Animal Cells, John Wiley & Sons, Inc., 2010. ISBN 978-0-470-52812-9.
-
LANZA R. ROBERT P. LANGER S. VACANTI J. ed. Principles of tissue engineering. 4th ed.. Amsterdam: Academic Press, 2014. ISBN 978-0123983589.
-
Lodish H. at al. Molecular Cell Biology. W.H. Freeman, 2013. ISBN 978-1-4641-0981-2.
-
RATNER B. D. ed. Biomaterials science: an introduction to materials in medicine. 3rd ed.. Amsterdam; Boston: Elsevier/Academic Press, 2013. ISBN 978-0123746269.
|