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Lecturer(s)
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Jenčová Věra, doc. Ing. Ph.D.
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Course content
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Lectures: <ol> <li> Introduction to cell biology: chemical and molecular principles. </li> <li> Proteins: hierarchical structure, folding, denaturation. </li> <li> Proteins: function, structure-function relationship, enzymes. </li> <li> Basic mechanisms of genetics: structure and function of nucleic acids. </li> <li> Basic mechanisms of genetics: transfer of genetic information (transcription, translation). </li> <li> Protein synthesis, gene expression control. </li> <li> Methods: principles of methods used for protein analysis (enzyme reactions, immunological methods). </li> <li>. Methods: principles of methods used for nucleic acid analysis (PCR, sequencing, cloning). </li> <li> Structure and function of prokaryotic cell. </li> <li> Structure and function of eukaryotic cells. </li> <li> Biological membrane: structure, electrochemical potential. </li> <li> Transport of substances through the biological membrane. </li> <li> Cell life cycle (metabolism, proliferation, differentiation, death). </li> <li> Integration of cells into tissues: intercellular matter, intercellular junctions, signals. </li> </ol> Seminars: Laboratory exercises are focused on practical exercises and verification of students' knowledge gained during lectures and study of recommended literature. <ul> <li> protein work (isolation and analysis): cell lysis, centrifugation (separation), quantitative Bradford analysis (spectrophotometry), electrophoresis (SDS-PAGE), enzyme assay (HRP, spectrophotometry), </ li > <li> DNA work (isolation and analysis): column isolation, phenol-chloroform extraction, quantification (spectrophotometry, pico green - fluorimentrie), qualitative analysis: PCR, electrophoresis (agarose), </li> <li> working with cells: cultivation of tissue cultures, microscopy of native, fixed, stained cells and cell elements (optical, fluorescent). </li> </ul>
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Learning activities and teaching methods
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Project teaching, Laboratory work, Problematic methods (research and exploration), Lecture
- Preparation for laboratory testing; outcome analysis
- 14 hours per semester
- Preparation for exam
- 14 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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The course provides students with basic information concerning cell biology and tissue biology. Lectures and practical laboratory exercises will cover the basic principles of the structure and function of biomacromolecules, the structure and life cycle of cells. Furthermore, students will be introduced to the principles of methods used in molecular biology. Learning outcomes of the course unit The subject will provide basic information that is necessary for the student's scientific background and understanding of teaching in the following subjects. Laboratory exercises are focused on practical exercises and verification of students' knowledge gained during lectures and study of recommended literature.
Theoretical knowledge and practical skills in the field.
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Prerequisites
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knowledge of secondary school biology
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Assessment methods and criteria
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Combined examination, Written assignment
Credit: Laboratory exercise 100% attendance, elaboration of protocols. Combined exam: oral and written part.
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Recommended literature
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Alberts B., Bray D. a kol. Základy buněčné biologie, Espero Publishing.. Ústí n.L, 2001.
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Freshney I. R. Culture of Animal Cells, John Wiley & Sons, Inc., 2010. ISBN 978-0-470-52812-9.
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Lodish H. at al. Molecular Cell Biology. W.H. Freeman, 2013. ISBN 978-1-4641-0981-2.
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