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Lecturer(s)
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Klíčová Markéta, Ing. Ph.D.
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Horáková Jana, doc. RNDr. Ph.D.
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Course content
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Lectures 1. Introduction to cell biology, microscopic methods (optical, fluorescent, electron) 2. Chemical composition of the cell 3. Structure and function of the cell ? prokaryote, eukaryote 4. Cell organelles 5. Transport of substances through membranes, communication between cells 6. Life cycle of a cell 7. Cell cultures 8. Tissues 9. Blood cells and their use in tissue engineering 10. Wound healing, reaction of the organism to biomaterial Exercises 1. Safety in the biological laboratory, basics of pipetting, terminology of cell culture laboratory equipment 2. Optical microscopy - observation of cells and visible cell structures 3. Fluorescent staining I ? fixation of cells, staining with fluorescent dyes (highlighting of the cell nucleus), observation, evaluation of the number of cells 4. Fluorescent staining II - fixation of cells, staining with fluorescent dyes (highlighting of the cell nucleus), observation, evaluation of the extent of cell proliferation 5. Counting cells in suspension ? Burker's chamber / analyzer to determine the number of viable cells in suspension (trypan blue) 6. Cell passaging 7. Spectrophotometry - colorimetric tests (cck-8) - carrying out a metabolic test, measuring absorbance, constructing a calibration line 8. Metabolic test evaluation ? data processing, linearity verification, discussion of the dispersion of measured values
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Learning activities and teaching methods
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Monological explanation (lecture, presentation,briefing), Self-study (text study, reading, problematic tasks, practical tasks, experiments, research, written assignments), Laboratory work, E-learning
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Learning outcomes
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The course will provide to students basic information regarding cell biology, the structure and function of tissues. The structure and function of biomacromolecules, the organization of cells, intercellular matter and tissues are explained in the lectures. During practical exercises, students are introduced to work in clean rooms, they learn to work with cell cultures. The subject provides basic information necessary for the student's natural science foundation and understanding of the teaching of related subjects (Fibrous materials for tissue engineering MAT, Tissue engineering MTI, Medical textiles ZDT).
Theoretical knowledge and practical skills in the field.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Combined examination, Oral exam, Didactic test
Requirements: Credit: completion of practical exercises, preparation of protocols Exam: written + oral
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Recommended literature
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Alberts B. et al. Základy buněčné biologie: úvod do molekulární biologie buňky. Ústí n. L., Espero Publishing, 1998. ISBN 8090290620.
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NEČAS, Oldřich. Obecná biologie pro lékařské fakulty. 3. přeprac. vyd., V nakl. H & H 1. vyd. Jinočany: H & H, 2000. ISBN 80-86022-46-3. ISBN 80-86022-46-3.
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ŠEBEK, Jaroslav. Buněčné kultury v medicíně. Praha: Galén, 2018.. ISBN 978-80-7492-380-7.
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