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Lecturer(s)
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Lubasová Alena, MUDr. EuSpLM
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Hradiská Jana, RNDr. Ph.D.
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Course content
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Lectures: 1) Organization of clinical biochemistry, safety of work in clinical biochemistry, preanalytical effects on the results of laboratory examination 2) Metabolism of basic nutrients, amino acids and disorders of their metabolism, carbohydrates, lipids, proteins, nucleotides, enzymes 3) Urine examination (physical examination, chemical examination, urinary sediment examination; renal function examination) 4) Examination of acid-base balance, metabolism of ions; liver function tests; diabetes mellitus, oGTT, self monitoring, blood glucose analysis 5) Hyperlipidemia, cholesterol determination; enzyme testing, cardiomarkers, oncogenic markers, hormone testing; proteins, acute inflammation, CSF analysis, molecular biology methods, PCR analysis Exercises: 1) Good laboratory practice in biochemistry 2) Collection, transport and examination of biological material: urine examination 3) Collection, transport and examination of biological material: examination of ABR, ions, liver function, diabetes mellitus, self monitoring 4) Collection, transport and examination of biological material: lipids, cholesterol, enzymes, cardiomarkers, oncogenic markers, hormones, proteins and others 5) Biochemical monitoring of patients in acute and resuscitation care - bedside testing including practical demonstration
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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The course is designed as a theoretical-practical unit. It acquaints students with basic knowledge of biochemistry, especially metabolism of basic nutrients, amino acids and disorders of their metabolism, carbohydrates, lipids, proteins, nucleotides, enzymes. Students acquire knowledge about biological material for clinical-biochemical examination, methods and techniques of sampling, marking of samples, their storage and sending for processing to clinical-biochemical laboratories and with reference values. It introduces basic technologies for biochemical examinations.
Students obtain knowledge in given course in accordance with requirements and course programme.
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Prerequisites
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Preconditions are frased in the annotation of the course and in the curriculum of the studying programme.
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Assessment methods and criteria
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unspecified
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Recommended literature
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DASTYCH, Milan a Petr BREINEK. Klinická biochemie, bakalářský obor Zdravotní laborant. Brno: Masarykova Univerzita, Lékařská fakulta, 2015. ISBN 978-80-210-4572-9.
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KOOLMAN, Jan a Klaus-Heinrich RÖHM. Barevný atlas biochemie. Praha: Grada, 2012. ISBN 978-80-247-2977-0.
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MATOUŠ, Bohuslav. Základy lékařské chemie a biochemie. Praha: Galén, 2010. ISBN 978-80-7262-702-8.
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POKORNÁ, Andrea a kol. Ošetřovatelské postupy založené na důkazech: 2. díl. Brno: Masarykova univerzita, 2019. ISBN 978-80-210-9297-6.
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RIFAI, Nader. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. St. Louis: Saunders, 2017. ISBN 9780323359214.
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ŘEZÁČOVÁ, Martina a Alena STOKLASOVÁ. Základy biochemie lidského organismu. Praha: Karolinum, 2012. ISBN 978-80-246-2149-4.
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ŠTERN, Petr. Obecná a klinická biochemie pro bakalářské obory studia. Praha: Karolinum, 2011. ISBN 978-80-246-1979-8.
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