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Lecturer(s)
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Jenčová Věra, doc. Ing. Ph.D.
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Strnadová Kateřina, Mgr. Ph.D.
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Course content
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Lectures: 1. Introduction to the cell: cell chemistry and biosynthesis 2. Protein structure 3. Protein function 4. Nucleic acids, chromosomes, genomes 5. Basic molecular genetics mechanisms (how cells read the genome) 6. Manipulating proteines, nucleic acids 7. Molecular genetics techniques 8. Internal organisation of the cell 9. Membrane structure, function 10. The cell cycle 11. Cells in their social context (cell junctions, cell adhession, extracellular matrix, signals) Practical courses: 1st and 2nd: work with proteins (isolation and analysis): cell lysis, centrifugation, quantitative analysis by Bradford method (spectrophotometry), electrophoresis (SDS-PAGE), enzyme assay (HRP) 3rd and 4th: work with DNA (isolation and analysis): column isolation, phenol-chloroform extraction, quantification (spectrophotometry, pico green - fluorimetry), qualitative anaysis: PCR, electrophoresis (agarose)
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Learning activities and teaching methods
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Monological explanation (lecture, presentation,briefing)
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Learning outcomes
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The course will provide students with basic information about biology of the cell and tissues. In the lectures and practical laboratory exercises the basic principles concerning structure and function of biomacromolecules, structure and life cycle of cells, structure and function of tissues will be discussed including biomechanics of selected tissues. Students will be acquainted with principles of methods used in molecular biology incl. working with living cell cultures. The course will provide the basic information necessary for the student's natural background and the understanding of teaching in related subjects (Tissue Engineering, Medical Textiles, Fibrous Materials for Tissue Engineering). Laboratory exercises are focused on practical exercises and verification of students' knowledge gained during lectures and study of recommended literature.
To explain and deduce basic knowledge on field, including simpler problem solving.
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Prerequisites
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knowledge of high school physics and biology
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Assessment methods and criteria
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Combined examination
knowledge of high school physics and biology
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Recommended literature
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Alberts B., Bray D. a kol. Základy buněčné biologie, Espero Publishing.. Ústí n.L, 2001.
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Alberts B, et al. Molecular Biology of the cell. 5th ed.. 2008. ISBN 978-0-8153-4110-9.
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Lodish H. at al. Molecular Cell Biology. W.H. Freeman, 2013. ISBN 978-1-4641-0981-2.
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